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Channel Mapping Calibration
Channel Mapping Calibration is a complex non-linear calibration process, which is required for multi-color imaging (e.g. co-localization). Calibration Instructions Connect to the microscope, start the temperature control, and wait until the ...
Camera calibration with NimOS 19.7 (and above)
The procedure takes around 10 min INSTRUCTIONS: 1- Log in the Nanoimager laptop with an administrator account 2- If not already done, Download CODI Acquisition from the following link (1gb) here 3- Install CODI Acquisition and accept every prompt ...
Function: 3D
The 3D function uses a cylindrical lens to introduce a specific astigmatism pattern for 3D super-resolution imaging (not applicable to confocal or widefield). For detailed instructions on how to perform 3D Mapping Calibration, check here. ...
Prepare Bead Slide
A bead slide is an essential part of everyday imaging routines as they are used for Channel Mapping Calibration during multiplex 2D imaging and 3D Mapping Calibration for 3D super-resolution imaging. ONI uses 100 nm beads routinely for these ...
AutoEV and AutoLNP Troubleshooting
Updating CODI System App (CSA) When prompted to update the CODI System app, it is critical that the update is performed before running an experiment. This update message appears when the CODI website has been updated but the CSA version on the ...