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Data Interpretation
The output format varies depending on how the acquisition is performed: Normal Acquisition Normal acquisition refers to taking images without using any advanced features (e.g. light program). Normally each normal acquisition generates 8 files: .locb: ...
Data Import
Instructions: In Analyze, click on Load Data. Navigate to the folder of interest and select either the Tiff file or the Locb File to import. Discussion: If the dataset is modified via 3rd party software such as ImageJ, it may not be compatible with ...
Data Export
There are different ways to save/export data from NimOS: Copy To Clipboard (Raw) Instructions: Right-click on any of the channels and click on Copy To Clipboard: Paste it into any other data viewer (e.g. ImageJ, Paint, Excel...) to visualize and save ...
NimOS Tabs
NimOS Tabs: Files: Load Data: open an acquisition, which will then appear in the Analyze interface. Instrument: Instrument Config: show general information about the microscope. Manual Connect: manually select an instrument file to connect to the ...
Function: Tracking
The Single-Molecule or Single-Particle Tracking function allows the observation of the motion of individual molecules/particles: For video instructions on how to use the tracking function, check here. Run Tracking Click on Tracking from Tools to open ...