Prepare EV Sample with EV profiler kit

The ONI's Extracellular Vesicle (EV) Profiler Kit provides a complete solution for single-EV imaging and analysis. This includes reagents and chips for EV labeling, capture, and imaging using antibodies against the tetraspanins CD81, CD63, and CD9 labeled with dSTORM compatible fluorophores, as well as a standard EV positive control. The CX team will organize at least one complete training session for the kit upon purchase.

To access our analysis or staining protocol, please Sign up and/or Sign up on this website, on the top right corner of this page. 

If at any point you are having issues with the preparation process, please do not hesitate to contact the CX team.

• Related Articles

• Prepare dSTORM Sample for cell imaging

  dSTORM is the "direct" variant of STORM that makes use of fluorophores that are very bright, have a high rate of photoswitching, and exhibit minimal photobleaching. Materials Cells must be plated on a high precision #1.5H coverslip or ibidi petri ...

• Prepare Bead Slide

  A bead slide is an essential part of everyday imaging routines as they are used for Channel Mapping Calibration during multiplex 2D imaging and 3D Mapping Calibration for 3D super-resolution imaging. ONI uses 100 nm beads routinely for these ...

• Preparing tissue sample for dSTORM imaging

  Preparing tissue sample for dSTORM imaging is not that different than preparing them for conventional microscopy. Recommendation: - Your sample can either be cryosection or Paraffin Embedded Tissues - FFPE. - If you have the choice we would recommend ...

• How to guide: Creating an EV or LNP Report

  The EV or LNP Report can be generated at any time in CODI using the “cross dataset report” feature, which provides a report for a single chip with up to 4 lanes. To generate a report, navigate to the CODI collaboration in which the data is saved and ...

• How to analyse data in CODI?

  First, for the moment CODI is only able to display super-resolution microscopy data, meaning images create from localisations of blinking event. For the visualisation or analysis of raw images, such as brightfield, widefield or tracking, please us ...