The Nanoimager works optimally with #1.5H coverslips, which have 170µm thickness.
The most popular chambers are:
ibidi (µ-Slide 8 Well Glass Bottom, No. 1.5) ]
Lab-Tek (Nunc™ Lab-Tek™ II Chambered cover-glass, No. 1.5)
35 mm petri dishes with #1.5 cover-glass bottom
The Nanoimager can heat from its working temperature (usually 27 to 30’C) and up to 42’C.
It can’t however cool down to a lower temperature.