NimOS Functions
Function: Multi-Acquisition
This Article contains information on mulitacquisition from various versions of Nanoimager software. Most of the functionalities remained the same but new functionalities and some changes have been introduced in user interface. Changes in Multi ...
Function: PythONI
What's PythONI? NimOS comes with its own python IDE called PythONI (/pi thon ni/). The simple python IDE allows you to write your own automation python script for controlling almost every part of the Nanoimager. This instruction will cover what's ...
Function: FRET
The FRET function allows measuring distances at a single-molecule scale. For video instructions on how to use the FRET function, check here. If at any point you are having issues with the FRET process, please do not hesitate to contact the CX team.
Function: Tracking
The Single-Molecule or Single-Particle Tracking function allows the observation of the motion of individual molecules/particles: For video instructions on how to use the tracking function, check here. Run Tracking Click on Tracking from Tools to open ...
Function: 3D
The 3D function uses a cylindrical lens to introduce a specific astigmatism pattern for 3D super-resolution imaging (not applicable to confocal or widefield). For detailed instructions on how to perform 3D Mapping Calibration, check here. ...
Function: Confocal
The Confocal function allows the acquisition of high-resolution confocal images, which is useful when dSTORM is not viable: Enable/Disable Confocal: enable/disable the confocal mode. Enable Navigation Mode: allow to quickly navigate the sample with ...
Function: TIRF
The TIRF function allows increasing the signal-to-noise ratio for super-resolution imaging. By default, the illumination on the Nanoimager is the same as epifluorescence wide-field microscopy. This corresponds to a degree of 0, in which light is ...
Function: Light Program
The Light Program function allows creation of a custom sequence of laser illumination for imaging: Total Number of Steps: add a different sequence of laser states to the light program. The maximum number of steps allowed is 5. Number of States: add a ...
Function: ROI
The ROI function allows cropping a smaller area for imaging and recording: Draw ROI: draw to select the area (rectangle). Clear ROI: clear the current selection. Instructions Click on ROI to expand the tab. Click on Draw ROI and draw a rectangle on ...
Function: Position List
The Open Position List function allows saving/reading positions to/from a list, which can be combined with the Overview Scan function for multi-position imaging: Load From File/Save To File: load/save a position list from/to a JSON file. Label For ...
Function: Overview Scan
The Overview Scan function allows acquiring a mosaic containing multiple positions for identifying the imaging positions (e.g. cell positions): Scan Parameters: set the origin and number of tiles to acquire for X and Y. Set Initial Position as ...
Function: Z Lock
The Nanoimager has a Z Lock functionality which allows for long and stable acquisitions from the desired focal plane. This feature is very useful for multiple types of imaging including dSTORM, tracking, overview scan, and multi-position ...