Sample Preparation
Labelling antibodies preparation
dSTORM requires specific dyes attached to antibodies, and while vendors have a large choice of conjugated antibodies, you may not be able to find the one you are looking for. The good news is that those same vendors are now proposing labelling kit, ...
Preparing clean coverslips
Clean coverslips are important in Single Molecule Localization Microscopy because any background noise present on the glass can be detected and localized. While those spots can usually be removed with analysis filters, it is always better to start ...
Preparing tissue sample for dSTORM imaging
Preparing tissue sample for dSTORM imaging is not that different than preparing them for conventional microscopy. Recommendation: - Your sample can either be cryosection or Paraffin Embedded Tissues - FFPE. - If you have the choice we would recommend ...
Prepare EV Sample with EV profiler kit
The ONI's Extracellular Vesicle (EV) Profiler Kit provides a complete solution for single-EV imaging and analysis. This includes reagents and chips for EV labeling, capture, and imaging using antibodies against the tetraspanins CD81, CD63, and CD9 ...
Prepare dSTORM Sample for cell imaging
dSTORM is the "direct" variant of STORM that makes use of fluorophores that are very bright, have a high rate of photoswitching, and exhibit minimal photobleaching. Materials Cells must be plated on a high precision #1.5H coverslip or ibidi petri ...
Prepare Bead Slide
A bead slide is an essential part of everyday imaging routines as they are used for Channel Mapping Calibration during multiplex 2D imaging and 3D Mapping Calibration for 3D super-resolution imaging. ONI uses 100 nm beads routinely for these ...
What slides/samples are compatible with the system?
The Nanoimager works optimally with #1.5H coverslips, which have 170µm thickness. The most popular chambers are: ibidi (µ-Slide 8 Well Glass Bottom, No. 1.5) ] Lab-Tek (Nunc™ Lab-Tek™ II Chambered cover-glass, No. 1.5) 35 mm petri dishes with #1.5 ...