Function: 3D

Function: 3D

The 3D function uses a cylindrical lens to introduce a specific astigmatism pattern for 3D super-resolution imaging (not applicable to confocal or widefield). For detailed instructions on how to perform 3D Mapping Calibration, check here.
Instructions
  1. Perform 3D Mapping Calibration.
  2. Click on Disengage 3D Lens and click on Engage 3D Lens again to apply the calibration.
  3. Set the desired acquisition parameters and click on Acquire to start the acquisition.
Discussions
  1. For more details on the technique, check here.
  2. The imaging depth is 1 um.

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