Image Display Control
Image Display Control:
- Channel 0 and/or Channel 1: select the channels for viewing and acquisition.
- Percentiles or Photon Counts: select one contrast method and set the corresponding threshold for viewing, in which the contrast for each channel is adjusted individually in real-time (Percentiles) or fixed (Photon Counts). The left column is for channel 0 and the right column is for channel 1. The Percentiles metric is similar to auto-contrast and therefore does not indicate the intensity relationship between the two channels. For example, the two channels can have the same visual looking but with completely different intensities, and therefore photobleaching of the sample is also not indicated. Interactive software for exploring the difference between the two methods can be downloaded here.
- Split Channels or Color Overlay: select one display method for viewing, in which the channels are displayed individually (Split Channels) or are merged into a single field of view (Color Overlay). In some systems, there is another display method (Raw Images), in which the full camera chip is displayed instead of the two cropped channels.
- Display Localization during Acquisition: show an axis-aligned bounding box around each localization during acquisition.
- Link/Unlink Channels: link/unlink the channels for setting thresholds
.
Related Articles
User Control
User Control: Click on Settings > User Settings to open the user window. Current User: set the user name for the current user. Each user has a unique acquisition setting and adding a new user creates a separate output folder in "C:/data/username" by ...Laser Control
Light Control: A and B: allow to have 2 global laser configurations. For example, put lower power in A for preview and put higher power in B for imaging. Laser Tab: turn on/off the laser. The laser is on when the tab is light and is off when the tab ...How to: Acquire confocal image
This is an How to....? guide For detailed information about the confocal control, please read that article. Line-scanning confocal is a new feature that is available in NimOS 1.16 software and above. This guide will cover - 1). How to acquire a ...NimOS Tabs
NimOS Tabs: Files: Load Data: open an acquisition, which will then appear in the Analyze interface. Instrument: Instrument Config: show general information about the microscope. Manual Connect: manually select an instrument file to connect to the ...Display troubleshooting guide
Important information about screen glitch following computer update on the Nanoimager laptop This note addresses a bug which came to our attention recently that can result in various resolution issues on the Nanoimager laptop. This seems to be ...