Laser Control

Light Control:

1. A and B: allow to have 2 global laser configurations. For example, put lower power in A for preview and put higher power in B for imaging.
   
2. Laser Tab: turn on/off the laser. The laser is on when the tab is light  and is off when the tab is dark . You can then use the slider to increase/decrease the laser power. The laser power percentage is shown in the box in % while the laser power reading (at the objective) is shown below the box in mW:
   
   
3. Transillumination: turn on/off the LEDs for transillumination at three different wavelengths (465 nm, 520 nm, and 620 nm). You can then use the slider to increase/decrease the LED power (not supported or disabled in some systems). The LED is on when the tab is light  and is off when the tab is dark .
   
4. Program: create/load a light program for automated acquisition of a single FOV. For more details, check here.
   
5. Focus Laser: turn on/off the focus laser. The focus laser is on when the tab is light  and is off when the tab is dark .
   
6. Disable Lasers/Enable Active Lasers: allow to turn on/off all the four imaging lasers at once (similar to safety switch).
   
7. Enable Light Program: activate the light program for image acquisition. If the camera is on, the light program will be triggered immediately. If the camera is off, the light program will be only triggered after the camera is turned on (e.g. start an acquisition). For more details on how to use the light program, click on the exclamation button .
8. Configure Illumination: customize the illumination pattern. For more instructions, please contact the CX team.

• Related Articles

• Temperature Control

  Temperature Control: Target: set the target temperature. All the thermistor readings can be monitored in the Microscope Box Temperature tab: Current: show the temperature feedback in real-time, averaged from all thermistors. Hover around the current ...

• Keyboard Control

  NimOS offers several keyboard controls. To activate the keyboard control, left-click on any of the channels. Arrows: controls X/Y translation. PgUp/PgDn: controls Z translation. Ctrl: activate coarse translation (faster speed). The speed can be ...

• Stage Control

  Stage Control: For instructions on how to control the stage using the keyboard, click on the exclamation button . Target Position: set the target stage position in mm. Hover around the target position box to show the preset translation range of each ...

• User Control

  User Control: Click on Settings > User Settings to open the user window. Current User: set the user name for the current user. Each user has a unique acquisition setting and adding a new user creates a separate output folder in "C:/data/username" by ...

• Acquisition Control

  Acquisition Control: A and B: allow to have 2 global acquisition configurations. For example, use A for taking an actual acquisition and B for taking a snapshot (one frame). Exposure (ms)/Frequency (Hz): set the exposure time or frequency for camera ...